This Program Announcement expires on October 5, 2004, unless reissued.

FUNCTIONAL TISSUE ENGINEERING OF MUSCULOSKELETAL TISSUES

Release Date:  October 18, 2001

PA NUMBER:  PA-02-014

National Institute of Arthritis and Musculoskeletal and Skin Diseases
National Institute of Child Health and Human Development
National Institute of Dental and Craniofacial Research

THIS PA USES "MODULAR GRANT" AND "JUST-IN-TIME" CONCEPTS.  MODULAR 
INSTRUCTIONS MUST BE USED FOR RESEARCH GRANT APPLICATIONS UP TO $250,000 PER 
YEAR. MODULAR BUDGET INSTRUCTIONS ARE PROVIDED IN SECTION C OF THE PHS 398 
(REVISION 5/2001) AVAILABLE AT 
http://grants.nih.gov/grants/funding/phs398/phs398.html.

PURPOSE

The purpose of this Program Announcement (PA) is to encourage the submission 
of applications for research to enhance our understanding of functional 
tissue engineering of musculoskeletal tissues (articular cartilage, 
ligaments, tendons, bone, meniscus, intervertebral disc and skeletal muscle).  
Innovative approaches to these scientific areas will be stressed.  

HEALTHY PEOPLE 2000

The Public Health Service (PHS) is committed to achieving the health 
promotion and disease prevention objectives of "Healthy People 2010," a PHS 
led national activity for setting priority areas.  This Program 
Announcement(PA), Functional Tissue Engineering of Musculoskeletal Tissues, 
is related to one or more of the priority areas.   Potential applicants may 
obtain a copy of "Healthy People 2010" at http://www.health.gov/healthypeople.

ELIGIBILITY REQUIREMENTS

Applications may be submitted by domestic and foreign, for-profit and non-
profit organizations, public and private, such as universities, colleges, 
hospitals, laboratories, units of State and local governments, and eligible 
agencies of the Federal government.  Racial/ethnic minority individuals, 
women, and persons with disabilities are encouraged to apply as principal 
investigators.

MECHANISM OF SUPPORT

The Research Project (R01) grant mechanism will be used to support projects 
under this PA.  Under this mechanism, the applicant will plan, direct, and 
carry out the research plan.  The proposed project period during which the 
research will be conducted should adequately reflect the time required to 
accomplish the stated goals and be consistent with the policy for grant 
support.  For R01s, the total requested project period may not exceed five 
years and applicants should apply for the length of time appropriate for the 
work proposed, typically three to five years.  NIDCR will allocate funds to 
support individual R01s, provided the applications are of high scientific 
merit.  It is possible that a collaborative project in a topic area that 
overlapped the interests of the NIAMS, NICHD and NIDCR could be co-funded by 
any combination of these three Institutes, if the project is of high 
scientific merit.

Specific application instructions have been modified to reflect "MODULAR 
GRANT" and "JUST-IN-TIME" streamlining efforts that have been adopted by the 
NIH. Complete and detailed instructions and information on Modular Grant 
applications have been incorporated into the PHS 398 (rev. 5/2001).  
Additional information on Modular Grants can be found at 
http://grants.nih.gov/grants/funding/modular/modular.htm.

Consistent with recommendations from the NIH Bioengineering Consortium, 
applications with a bioengineering perspective that are submitted in response 
to this PA may be either design/technology-driven, hypothesis-driven, or a 
combination of the two.

RESEARCH OBJECTIVES

Background

The goal of tissue engineering is to repair or replace tissues and organs by 
delivering implanted cells, scaffolds, DNA, proteins or protein fragments to 
areas where they are needed.  Despite early success, tissue engineers have 
faced challenges in repairing or replacing injured or diseased tissues that 
serve a predominantly biomechanical function, such as bone and articular 
cartilage.  An evolving discipline, “functional tissue engineering” seeks to 
address these challenges [Butler, et al, Functional Tissue Engineering: The 
Role of Biomechanics, Journal of Biomechanical Engineering, December 2000 
122(6), pages 570-575].  Principles of functional tissue engineering include 
the following: (1) In vivo stress/strain histories need to be measured for a 
variety of activities.  Such data provide mechanical threshholds that tissue 
repairs/replacements will likely encounter; (2) Mechanical properties of the 
native tissues must be established for sub-failure and failure conditions.  
These baseline data provide parameters within the expected threshholds for 
different in vivo activities and beyond these levels if safety factors are to 
be incorporated; (3) A subset of these mechanical properties must be selected 
and prioritized.  This subset is important, given that the mechanical 
properties of the designs are not expected to completely duplicate the 
properties of native tissues; (4) Standards must be set when evaluating the 
repairs/replacements after surgery to determine “how good is enough?”  New 
and improved methods must also be developed for assessing the function of 
engineered tissues; (5) The effects of physical factors on cellular activity 
must be determined in engineered tissues.  Knowing these signals may shorten 
the iterations required to successfully replace a tissue, and direct cellular 
activity and phenotype toward a desired end goal; and (6) To effect a better 
repair outcome, cell-matrix implants may benefit from being mechanically 
stimulated using in vitro “bioreactors” prior to implantation.  How 
mechanical stress, as well as other physical factors, influences cell 
activity in bioartificial matrices and bioreactors needs to be assessed.

Incorporating each of these principles of functional tissue engineering 
should result in safer and more efficacious repairs and replacements for the 
patient.  In addition, these “principles” outline scientific research 
opportunities that could vastly improve the scientific knowledge base and the 
result in tissue engineering applications for musculoskeletal injuries and 
diseases that involve musculoskeletal tissues (articular cartilage, 
ligaments, tendons, bone, meniscus, intervertebral disc and skeletal muscle). 

This PA is an outgrowth of the NIAMS, NICHD and NIDCR continued interest in, 
and support of, musculoskeletal tissue engineering research, and the National 
Institutes of Health BECON 2001 Symposium on Reparative Medicine: Growing 
Tissues and Organs.  Further information on this meeting can be found at:
http://www.becon.nih.gov/becon.htm.

Scope

The following are examples of research topics that are appropriate for this 
PA;  however, they are not to be considered as all inclusive or limiting:

Better understand and define the normal mechanical properties of the 
musculoskeletal tissues (i.e., the micro-environment) that we are trying to 
repair or replace.

Develop new and/or improved methods for the non-invasive measurement of 
mechanical function at the cell level and/or micro-environmental level.

Better understand the interactions between mechanical force and inflammatory 
processes with specific reference to musculoskeletal tissue engineered 
applications.

Better understand the intrinsic properties of extracellular musculoskeletal 
matrices (synthesis, characterization, fabrication; cell responsiveness; 
adaptation to the environment; and ways to improve marginal, inter-fascial 
attachments).

Better understand how mechanical stress, as well as other physical factors, 
influence cell activity in musculoskeletal bioartificial matrices and 
bioreactors.

Better understand the mechanical events that occur during rehabilitation 
following musculoskeletal tissue-engineered repair.

Identify and validate a minimum set of tests (in vitro and in vivo) to 
functionally validate engineered musculoskeletal tissues.

Develop and validate constitutive models of scaffold degradation and fatigue 
for use in musculoskeletal tissue engineering.

Develop and validate better mathematical (non-linear, time dependent) and 
computational (robust, finite element) models for use in musculoskeletal 
tissue engineering.

Better understand mechanical factors and their interaction with construct 
design parameters in musculoskeletal tissue engineering.

Develop and/or validate minimally-invasive imaging modalities to assess the 
progress/success of musculoskeletal tissue-engineered constructs.

Develop and/or validate other non-imaging-related, minimally-invasive 
modalities (i.e, biochemical, serologic, ultrasound, etc.) to assess the 
progress/success of musculoskeletal tissue-engineered constructs.

As science in this area advances, ultimately develop randomized, controlled 
outcome studies of the use of tissue-engineered constructs for the reparative 
medical treatment of musculoskeletal injuries and diseases.

INCLUSION OF WOMEN AND MINORITIES IN RESEARCH INVOLVING HUMAN SUBJECTS

It is the policy of the NIH that women and members of minority groups and 
their sub-populations must be included in all NIH-supported biomedical and 
behavioral research projects involving human subjects, unless a clear and 
compelling rationale and justification are provided indicating that inclusion 
is inappropriate with respect to the health of the subjects or the purpose of  
the research.  This policy results from the NIH Revitalization Act of 1993 
(Section 492B of Public Law 103-43). 

All investigators proposing research involving human subjects should read the 
UPDATED "NIH Guidelines for Inclusion of Women and Minorities as Subjects in 
Clinical Research," published in the NIH Guide for Grants and Contracts on 
August 2, 2000 
(http://grants.nih.gov/grants/guide/notice-files/NOT-OD-00-048.html); 
a complete copy of the updated Guidelines is available at  
http://grants.nih.gov/grants/funding/women_min/guidelines_update.htm.  The 
revisions relate to NIH defined Phase III clinical trials and require: a) all 
applications or proposals and/or protocols to provide a description of plans 
to conduct analyses, as appropriate, to address differences by sex/gender 
and/or racial/ethnic groups, including subgroups if applicable; and b) all 
investigators to report accrual, and to conduct and report analyses, as 
appropriate, by sex/gender and/or racial/ethnic group differences.
 
INCLUSION OF CHILDREN AS PARTICIPANTS IN RESEARCH INVOLVING HUMAN SUBJECTS

It is the policy of NIH that children (i.e., individuals under the age of 21) 
must be included in all human subjects research, conducted or supported by 
the NIH, unless there are scientific and ethical reasons not to include them.  
This policy applies to all initial (Type 1) applications submitted for 
receipt dates after October 1, 1998.

All investigators proposing research involving human subjects should read the 
"NIH Policy and Guidelines on the Inclusion of Children as Participants in 
Research Involving Human Subjects" that was published in the NIH Guide for 
Grants and Contracts, March 6, 1998, and is available at the following URL 
address: http://grants.nih.gov/grants/guide/notice-files/not98-024.html.

Investigators also may obtain copies of these policies from the program staff 
listed under INQUIRIES.  Program staff may also provide additional relevant 
information concerning the policy.

REQUIRED EDUCATION ON THE PROTECTION OF HUMAN SUBJECT PARTICIPANTS

NIH policy requires education on the protection of human subject participants 
for all investigators submitting NIH proposals for research involving human 
subjects.  This policy announcement is found in the NIH Guide for Grants and 
Contracts Announcement dated June 5, 2000, at the following website: 
http://grants.nih.gov/grants/guide/notice-files/NOT-OD-00-039.html.

URLS IN NIH GRANT APPLICATIONS OR APPENDICES

All applications and proposals for NIH funding must be self-contained within 
specified page limitations.  Unless otherwise specified in an NIH 
solicitation, internet addresses (URLs) should not be used to provide 
information necessary to the review because reviewers are under no obligation 
to view the Internet sites.  Reviewers are cautioned that their anonymity may 
be compromised when they directly access an Internet site.

PUBLIC ACCESS TO RESEARCH DATA THROUGH THE FREEDOM OF INFORMATION ACT

The Office of Management and Budget (OMB) Circular A-110 has been revised to 
provide public access to research data through the Freedom of Information Act 
(FOIA) under some circumstances.  Data that are (1) first produced in a 
project that is supported in whole or in part with Federal funds and (2) 
cited publicly and officially by a Federal agency in support of an action 
that has the force and effect of law (i.e., a regulation) may be accessed 
through FOIA.  It is important for applicants to understand the basic scope 
of this amendment.  NIH has provided guidance at:
http://grants.nih.gov/grants/policy/a110/a110_guidance_dec1999.htm.

Applicants may wish to place data collected under this PA in a public 
archive, which can provide protections for the data and manage the 
distribution for an indefinite period of time.  If so, the application should 
include a description of the archiving plan in the study design and include 
information about this in the budget justification section of the 
application. In addition, applicants should think about how to structure 
informed consent statements and other human subjects procedures given the 
potential for wider use of data collected under this award.

APPLICATION PROCEDURES

The PHS 398 research grant application instructions and forms (rev. 5/2001) 
at http://grants.nih.gov/grants/funding/phs398/phs398.html are to be used in 
applying for these grants and will be accepted at the standard application 
deadlines (http://grants.nih.gov/grants/dates.htm) as indicated in the 
application kit.  This version of the PHS 398 is available in an interactive, 
searchable format. Although applicants are strongly encouraged to begin using 
the 5/2001 revision of the PHS 398 as soon as possible, the NIH will continue 
to accept applications prepared using the 4/1998 revision until January 9, 
2002. Beginning January 10, 2002, however, the NIH will return applications 
that are not submitted on the 5/2001 version.  For further assistance contact 
GrantsInfo, Telephone 301/435-0714, Email: GrantsInfo@nih.gov.

Applicants planning to submit an investigator-initiated new (type 1), 
competing continuation (type 2), competing supplement, or any amended/revised 
version of the preceding grant application types requesting $500,000 or more 
in direct costs for any year are advised that he or she must contact the 
Institute or Center (IC) program staff before submitting the application, 
i.e, as plans for the study are being developed.  Furthermore, the applicant 
must obtain agreement from the IC staff that the IC will accept the 
application for consideration for award.  Finally, the applicant must 
identify, in a cover letter sent with the application, the staff member and 
Institute or Center who agreed to accept assignment of the application.  

This policy requires an applicant to obtain agreement for acceptance of both 
any such application and any such subsequent amendment.  Refer to the NIH 
Guide for Grants and Contracts, March 20, 1998 at 
http://grants.nih.gov/grants/guide/notice-files/not98-030.html.

SPECIFIC INSTRUCTIONS FOR MODULAR GRANT APPLICATIONS

The modular grant concept establishes specific modules in which direct costs 
may be requested as well as a maximum level for requested budgets. Only 
limited budgetary information is required under this approach.  The 
just-in-time concept allows applicants to submit certain information only 
when there is a possibility for an award. It is anticipated that these 
changes will reduce the administrative burden for the applicants, reviewers 
and NIH staff.  The research grant application form PHS 398 (rev. 5/2001) at 
http://grants.nih.gov/grants/funding/phs398/phs398.html is to be used in 
applying for these grants, with modular budget instructions provided in 
Section C of the application instructions.  Applicants are permitted, 
however, to use the 4/1998 revision of the PHS 398 for scheduled application 
receipt dates until January 9, 2002.  If you are preparing an application 
using the 4/1998 version, please refer to the step-by-step instructions for 
Modular Grants available at 
http://grants.nih.gov/grants/funding/modular/modular.htm.  Additional 
information about Modular Grants is also available on this site.

The title and number of the program announcement must be typed on line 2 of 
the face page of the application form and the YES box must be marked.

Submit a signed, typewritten original of the application, including the 
Checklist, and five signed photocopies in one package to:

CENTER FOR SCIENTIFIC REVIEW
NATIONAL INSTITUTES OF HEALTH
6701 ROCKLEDGE DRIVE, ROOM 1040, MSC 7710
BETHESDA, MD  20892-7710
BETHESDA, MD  20817 (for express/courier service)

REVIEW CONSIDERATIONS

Applications will be assigned on the basis of established PHS referral 
guidelines.  Applications will be evaluated for scientific and technical 
merit by an appropriate scientific review group convened in accordance with 
the standard NIH peer review procedures.  As part of the initial merit 
review, all applications will receive a written critique and undergo a 
process in which only those applications deemed to have the highest 
scientific merit, generally the top half of applications under review, will 
be discussed, assigned a priority score, and receive a second level review by 
the appropriate national advisory council or board.

Review Criteria

The goals of NIH-supported research are to advance our understanding of 
biological systems, improve the control of disease, and enhance health.  In 
the written comments reviewers will be asked to discuss the following aspects 
of the application in order to judge the likelihood that the proposed 
research will have a substantial impact on the pursuit of these goals.  Each 
of these criteria will be addressed and considered in assigning the overall 
score, weighting them as appropriate for each application.  Note that the 
application does not need to be strong in all categories to be judged likely 
to have major scientific impact and thus deserve a high priority score.  For 
example, an investigator may propose to carry out important work that by its 
nature is not innovative but is essential to move a field forward.

(1) Significance:  Does this study address an important problem?  If the aims 
of the application are achieved, how will scientific knowledge be advanced?  
What will be the effect of these studies on the concepts or methods that 
drive this field?

(2) Approach:  Are the conceptual framework, design, methods, and analyses 
adequately developed, well-integrated, and appropriate to the aims of the 
project?  Does the applicant acknowledge potential problem areas and consider 
alternative tactics?

(3) Innovation:  Does the project employ novel concepts, approaches or 
method? Are the aims original and innovative?  Does the project challenge 
existing paradigms or develop new methodologies or technologies?

(4) Investigator:  Is the investigator appropriately trained and well suited 
to carry out this work?  Is the work proposed appropriate to the experience 
level of the principal investigator and other researchers (if any)?

(5) Environment:  Does the scientific environment in which the work will be 
done contribute to the probability of success?  Do the proposed experiments 
take advantage of unique features of the scientific environment or employ
useful collaborative arrangements?  Is there evidence of institutional 
support?

In addition to the above criteria, in accordance with NIH policy, all 
applications will also be reviewed with respect to the following:

o  The adequacy of plans to include both genders, minorities and their 
subgroups, and children as appropriate for the scientific goals of the 
research.  Plans for the recruitment and retention of subjects will also be 
evaluated.

o  The reasonableness of the proposed budget and duration in relation to the 
proposed research.

o  The adequacy of the proposed protection for humans, animals or the 
environment, to the extent they may be adversely affected by the project  
proposed in the application.

o  The adequacy of the proposed plan to share data, if appropriate.

AWARD CRITERIA

Award criteria that will be used to make award decisions include:
o scientific merit

o availability of funds
o programmatic priorities.

INQUIRIES

Inquiries are encouraged.  The opportunity to clarify any issues or questions 
from potential applicants is welcome.

Direct inquiries regarding programmatic issues to:

James S. Panagis, MD, MPH
Director, Orthopaedics Program
National Institute of Arthritis and Musculoskeletal and Skin Diseases
45 Center Drive, Room 5AS-37K
Bethesda, MD  20892-6500
Telephone:  (301) 594-5055
FAX:  (301) 480-4543
Email:  jp149d@nih.gov

Louis A. Quatrano, Ph.D.
Behavioral Sciences and Rehabilitation Engineering Program
National Center for Medical Rehabilitation Research, NICHD
6100 Executive Blvd. Rm. 2A-03
Bethesda, MD  20852
Telephone:  (301)402-4221
FAX:  (301) 402-0832
Email:  lq2n@nih.gov

Eleni Kousvelari, DDS, D.Sc.
Chief, Cellular and Molecular Biology
National Institute of Dental and Craniofacial Research
45 Center Drive, Room 4AN-18A
Bethesda, MD  20892-6500
Telephone:  (301) 594-2427
FAX:  (301) 480-8318
Email:  kousvelari@de45.nidr.ni.gov

Direct inquiries regarding fiscal matters to:

Ms. Melinda Nelson
Grants Management Office
National Institute of Arthritis and Musculoskeletal and Skin Diseases
45 Center Drive, Room 5AS-49F
Bethesda, MD  20892-6500
Telephone:  (301) 594-3535
FAX:  (301) 480-5450
Email:  nelsonm@mail.@nih.gov

Christopher Myers
Grants Management
NICHD
6100 Executive Blvd. Rm. 8A17
Bethesda, MD  20852
Telephone:  (301)435-6966
FAX:  (301) 402-0915
Email:  myersc@mail.nih.gov

Martin R. Rubenstein
Chief Grants Management Officer
National Institute of Dental and Craniofacial Research
Telephone:  (301) 594-4800
FAX:  (301) 480-8301
Email:  Martin.Rubenstein@NIH.GOV

AUTHORITY AND REGULATIONS 

This program is described in the Catalog of Federal Domestic Assistance No. 
93.846, 93.929 and 93.158.  Awards are made under authorization of the Public 
Health Service Act, Title IV, Part A (Public Law 78-410, as amended by Public 
Law 99-158, 42 USC 241 and 285) and administered under PHS grants policies 
and Federal Regulations 42 CFR 52 and 45 CFR Part 74.  This program is not 
subject to the intergovernmental review requirements of Executive Order 12372 
or Health Systems Agency review.

The PHS strongly encourages all grant and contract recipients to provide a 
smoke-free workplace and promote the non-use of all tobacco products.  In 
addition, Public Law 103-227, the Pro-Children Act of 1994, prohibits smoking 
in certain facilities (or in some cases, and portion of a facility) in which 
regular or routine education, library, day care, health care or early 
childhood development services are provided to children.  This is consistent 
with the PHS mission to protect and advance the physical and mental health of 
the American people.


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