PREPARATION OF RADIOLABELED SPHINGOLIPIDS

NIH GUIDE, Volume 21, Number 43, November 27, 1992



RFP AVAILABLE:  NIH-NINDS-93-03



P.T. 34



Keywords:

  Chemical Synthesis 

  Chemistry, Organic 



National Institute of Neurological Disorders and Stroke



The National Institute of Neurological Disorders and Stroke (NINDS),

NIH, has a requirement for the preparation and delivery of certain

synthesized radiolabeled sphingolipids needed to support basic and

clinical investigations of Gaucher's disease, Niemann-Pick disease,

and Fabry's disease conducted by staff of the Division of Intramural

Research, NINDS.



The Contractor shall be required to prepare and deliver:  (a) a total

of 3.0 grams of 14-C-glucocerebroside (1.0 gram during the basic

contract period, and 1.0 gram during each option period) specifically

labeled in the D-glucose portion of the molecule with a minimum

specific activity of 1000 d.p.m. per nanomole; (b) 1.0 gram of

14-C-sphingomyelin (to be delivered during the first option period)

labeled in the choline portion of the molecule with a minimum

specific activity of 1000 d.p.m. per nanomole; and (c) 1.0 gram of

14-C-ceramidetrihexoside (to be delivered during the second option

period) labeled exclusively in the terminal molecule of galactose

with a minimum specific activity of 1000 d.p.m. per nanomole.  The

labeled sphingolipids shall be synthesized with radiochemical purity

and in the form of the naturally occurring stereoisomers.  The

homogeneity of each sphingolipid delivered shall be documented by

elemental analysis, various physical methods including infrared, NMR,

and mass spectroscopy, thin-layer or high-pressure liquid

chromatography.  The specific radioactivity of the various compounds

shall be certified.



Offerors will be required to provide evidence of their competence,

ability, and prior experience in the synthesis of radioactive

sphingolipids.



This requirement represents a recompetition of work currently being

performed under NINDS Contract No. N01-NS-0-2386, with Lipitek, Inc.

It is expected that the incumbent Contractor will recompete.



One award is anticipated.  Award will be made on a fixed-price per

deliverable basis.  The basic contract will cover a 12-month term and

will contain two fixed-price, one year options.



This is not a Request for Proposals (RFP).  RFP No. NIH-NINDS-93-03

will be issued on or about December 1, 1992, with January 29, 1993,

set as a tentative closing date for receipt of proposals.



To receive a copy of the RFP, submit a written request to the

following address, and supply two self-addressed mailing labels:



Contracts Management Branch, DEA

National Institute of Neurological Disorders and Stroke

Federal Building, Room 901

7550 Wisconsin Avenue

Bethesda, MD  20892



Reference:  RFP No. NIH-NINDS-93-03



This announcement does not commit the NINDS to make an award.  All

responsible sources may submit a proposal that will be considered by

the agency.



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