CONTINUATION OF NIH BACTERIAL ARTIFICIAL CHROMOSOME (BAC) LIBRARY 
PRODUCTION PROGRAM – REQUEST FOR INFORMATION

RELEASE DATE:  August 12, 2004

NOTICE:  NOT-HG-04-003

National Human Genome Research Institute (NHGRI)
 (http://www.genome.gov)

Purpose: The NHGRI is currently assessing the future of the genomic BAC 
library production program that is part of the NIH BAC Resource Network 
(http://genome.gov/10001844) and is, therefore, requesting information 
from researchers about their awareness of the program, research for 
which BAC libraries produced through the program have been used, 
utility of genomic BAC libraries in general, and assessment of future 
needs for BAC libraries.

Background: The bacterial artificial chromosome (BAC) is a vector 
system of choice for the construction of the large-insert chromosomal 
DNA libraries that are needed in genomic studies. The BAC cloning 
system is more stable than other cloning systems and allows the 
isolation of genomic DNA fragments that are large enough (80 to >200 
kilobases [kb]) to be useful for both targeted genomic sequence 
determination and for a variety of functional studies. In 2002, three 
centers were funded for three years 
(http://grants.nih.gov/grants/guide/rfa-files/RFA-HG-01-002.html) to 
produce genomic BAC libraries from multiple organisms and to increase 
the national capacity for BAC library construction. Both of these goals 
have been met.

The centers were funded as a production capacity without any decisions 
of particular organisms (excluding eubacteria, archea and plants) for 
which to make BAC libraries. In order to optimally select organisms, 
the research community was asked to propose species and present a 
rationale for library construction. Organisms were then chosen based on 
criteria such as medical relevance and uses for which the BAC library 
would be put. Through this process 86 organisms have entered the 
library production pipeline. Library quality is continuously assessed 
using a standard set of criteria (http://genome.gov/10001738) including 
average insert size and depth of genome coverage. The libraries are 
also screened for contamination. Libraries are currently available for 
36 species (http://genome.gov/10001852).

Information Requested: This Request for Information is an attempt to 
solicit information from the scientific community to better assess the 
need for future BAC libraries. Information in the following areas will 
aid in planning future BAC library construction capacity:

1) Are you aware of the existence of this program and the opportunity 
for researchers to propose organisms for BAC library production? If so, 
have you contributed to a white paper proposing an organism? Would you 
consider doing so in the future?

2) Have you used a BAC clone or library (generated through this program 
or otherwise) in the course of your research? If so, what was the 
source and quality of the library and can you please briefly describe 
how it was used? Did it facilitate your research and if so, how?

3) Are there still unmet needs for BAC libraries?  If yes, please list 
the organisms for which you would like to have BAC libraries 
constructed and state why these organisms are important to study.

This Request for Information is for information and planning purposes 
only and shall not be construed as a solicitation or as an obligation 
on the part of the NHGRI. The NHGRI does not intend to award a grant or 
contract on the basis of responses nor otherwise pay for the 
preparation of any information submitted or the Government's use of 
such information. Acknowledgment of receipt of responses will not be 
made, nor will respondents be notified of the Institute's evaluation of 
the information received. Responses will be held in a confidential 
manner. Any proprietary information should be so marked after each 
response.

Comments should be submitted via email to 
BAC_Library_Requests@mail.nih.gov by Friday, September 3, 2004.

Direct your questions to:

Kris Wetterstrand, M.S.
National Human Genome Research Institute
5635 Fishers Lane Suite 4076, MSC 9305
Bethesda, MD 20892-9305
Direct:   301-435-5543
Main:   301-496-7531
Fax:   301-480-2770
Email:   wettersk@mail.nih.gov


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